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Table 1 The summarised information of 12 potential HKGs based on transcriptome data

From: Transcriptome-based selection and validation of optimal house-keeping genes for skin research in goats (Capra hircus)

Type

Gene symbol

Mean_FPKM

CV (%)

Ranking order a

MFC b

DPM c

New predicted candidate HKGs

RRAGA

51.4

8.4%

6

1.416

0.083

PTPRA

23.8

9.1%

8

1.474

0.090

SRP68

27.2

9.2%

9

1.510

0.091

EIF4H

133.0

9.5%

16

1.479

0.094

NCBP3

10.0

9.5%

17

1.542

0.094

CTBP2

22.5

9.9%

25

1.566

0.098

CNBP

226.5

14.3%

458

1.880

0.141

EEF2

499.7

15.1%

619

1.923

0.149

Suggested by previous study

SDHA

44.0

18.5%

1679

2.710

0.182

YWHAZ

137.5

19.2%

1946

2.320

0.189

Conventional HKGs

ACTB

556.1

24.6%

4456

2.962

0.239

GAPDH

391.6

29.9%

6855

2.945

0.286

  1. a Ranking order in all genes based on CV value within all 15,853 unigenes
  2. b MFC, maximum fold change, highest/lowest FPKM value of one gene within 39 transcriptome profiles
  3. c DPM, dispersion measure, were determined by PaGeFinder method and an acceptable value should be ≤0.3
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BMC Genomics

ISSN: 1471-2164

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