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Table 1 The summarised information of 12 potential HKGs based on transcriptome data

From: Transcriptome-based selection and validation of optimal house-keeping genes for skin research in goats (Capra hircus)

Type Gene symbol Mean_FPKM CV (%) Ranking order a MFC b DPM c
New predicted candidate HKGs RRAGA 51.4 8.4% 6 1.416 0.083
PTPRA 23.8 9.1% 8 1.474 0.090
SRP68 27.2 9.2% 9 1.510 0.091
EIF4H 133.0 9.5% 16 1.479 0.094
NCBP3 10.0 9.5% 17 1.542 0.094
CTBP2 22.5 9.9% 25 1.566 0.098
CNBP 226.5 14.3% 458 1.880 0.141
EEF2 499.7 15.1% 619 1.923 0.149
Suggested by previous study SDHA 44.0 18.5% 1679 2.710 0.182
YWHAZ 137.5 19.2% 1946 2.320 0.189
Conventional HKGs ACTB 556.1 24.6% 4456 2.962 0.239
GAPDH 391.6 29.9% 6855 2.945 0.286
  1. a Ranking order in all genes based on CV value within all 15,853 unigenes
  2. b MFC, maximum fold change, highest/lowest FPKM value of one gene within 39 transcriptome profiles
  3. c DPM, dispersion measure, were determined by PaGeFinder method and an acceptable value should be ≤0.3