Fig. 9
From: The alcohol dehydrogenase gene family in sugarcane and its involvement in cold stress regulation
Spot assays used for monitoring the growth performance of BL21/pEZYHb and BL21/pEZYHb-ScADH3 transformed E. coli cells on LB plates under cold stress. The growth performance of BL21/pEZYHb and BL21/ pEZYHb-ScADH3 cells on LB plates without supplements or stresses were used as controls. After spotting the sample on LB agar plates, incubation at 4 °C in darkness for 3 days, 7 days, and 14 days was used to study the tolerance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells under cold stress