Proteomic responses to progressive dehydration stress in leaves of chickpea seedlings

Vessal, Saeedreza; Arefian, Mohammad; Siddique, Kadambot H. M.

doi:10.1186/s12864-020-06930-2

BMC Genomics

Table 1 Proteins from drought-treated chickpea with ≥1.5-fold alteration, relative to control values, identified by LC-MS/MS analysis and Mascot database search

From: Proteomic responses to progressive dehydration stress in leaves of chickpea seedlings

Spot no.^a	Protein identity	Species^b	Coverage (%)^c	MASCOT score^d	TM_r/EM_r^e	TpI/EpI^f	Acc. No.^g
	Photosynthesis and photorespiration
3	RuBisCO large subunit-binding protein subunit beta	Cicer arietinum	44	1137	62.9/59.2	5.85/5.53	XP_012567814.1
59	Probable ribose-5-phosphate isomerase 3, chloroplastic	Cicer arietinum	29	654	30.5/21.7	6.00/4.88	XP_004494688.1
66	Ribulose-bisphosphate carboxylase large subunit, partial	Salix bebbiana	18	55	23.5/14.5	5.61/4.94	AIG57943.1
7	Glycine dehydrogenase (decarboxylating), mitochondrial	Cicer arietinum	26	633	115.3/60.2	7.99/6.43	XP_004498896.2
33	ATP synthase subunit beta, chloroplastic	Cicer arietinum	39	585	52.9/28.7	5.16/4.99	B5LMK9
56	ATP synthase subunit alpha, chloroplastic	Cicer arietinum	54	321	19.4/21.5	5.05/5.16	B5LMN1
38	Fructose-bisphosphate aldolase 1, chloroplastic	Cicer arietinum	21	1376	43.2/33.5	6.28/5.98	XP_004507507.1
45	Carbonic anhydrase, chloroplastic isoform X2	Cicer arietinum	19	409	35.8/22.3	6.61/6.66	XP_004489275.1
49	psbP domain-containing protein 1, chloroplastic	Cicer arietinum	65	643	28.6/20.5	8.89/5.79	XP_004494530.1
22	ATP synthase beta subunit, partial (chloroplast)	Eucryphia lucida	50	655	51.8/40.2	5.20/5.38	AAK72764.1
32	Phosphoribulokinase	Erythranthe guttata	13	148	45.5/33.1	5.86/5.20	A0A022QP63
50	Chlorophyll a-b binding protein 3, chloroplastic	Cicer arietinum	22	511	29.4/20.7	6.32/5.56	XP_004491629.1
57	Oxygen-evolving enhancer protein 2, chloroplastic	Cicer arietinum	29	721	28.7/19.0	5.65/5.55	XP_004499534.1
62	Oxygen-evolving enhancer protein 1, chloroplastic	Cicer arietinum	45	679	34.9/19.0	6.24/4.83	XP_004509219.1
67	Oxygen-evolving enhancer protein 2, chloroplastic	Cicer arietinum	30	643	28.7/14.2	6.90/5.02	XP_004499534.1
73	Oxygen-evolving enhancer protein 2, chloroplastic	Cicer arietinum	16	117	28.7/9.25	6.90/4.72	XP_004499534.1
	Stress responsive
11	Heat shock protein 70	Chrysanthemum morifolium	13	265	70.8/42.2	5.12/4.86	A0A0A1HAD2
41	L-ascorbate peroxidase, cytosolic	Cicer arietinum	37	243	27.1/23.2	5.65/ 5.94	XP_004505943.1
51	L-ascorbate peroxidase, cytosolic	Cicer arietinum	57	781	27.1/20.75	5.65/5.36	XP_004505943.1
42	Glutathione s-transferase	Cicer arietinum	34	270	25.6/21.8	6.04/6.12	A0A0X9LEN0
77	Superoxide dismutase [Cu-Zn]	Solanum chacoense	15	1057	22.4/14.5	6.08/5.96	A0A0V0HK97
81	Cold shock protein	Cicer arietinum	31	1497	19.2/18.5	6.29/6.55	A0A088FZS5
	Protein synthesis and degradation
13	Elongation factor Tu, mitochondrial	Cicer arietinum	30	272	49.1/40.2	6.58/6.44	XP_004493639.2
17	Peptidyl-prolyl cis-trans isomerase CYP38, chloroplastic	Cicer arietinum	9	82	50.1/39.5	5.12/4.68	XP_004489294.1
10	Uncharacterized protein LOC101507383, partial, homoluguse with protein disulfide isomerase (query coverage 99%)	Cicer arietinum	15	152	42.7/46.2	4.83/4.81	XP_004495295.1
5	ATP-dependent zinc metalloprotease FTSH 2	Cicer arietinum	7	88	74.7/62.7	5.60/5.34	XP_004504668.1
8	Ubiquitin receptor RAD23d-like	Cicer arietinum	10	55	40.3/33.7	4.39/4.53	XP_004489115.1
	Gene transcription
37	33 kDa ribonucleoprotein, chloroplastic	Glycine soja	3	66	30.4/31.5	8.67/4.98	A0A0B2SU82
61	28 kDa ribonucleoprotein, chloroplastic	Cicer arietinum	26	642	29.8/24.5	4.74/4.64	XP_012570426.1
54	29 kDa ribonucleoprotein A, chloroplastic	Cicer arietinum	16	110	30.7/23.2	5.36/4.83	XP_004497514.1
76	U3 small nucleolar ribonucleoprotein protein MPP10-like isoform	Malus domestica	1	61	62.3/13.2	4.5/5.6	XP_008367517.1
	Signaling
47	Auxin-binding protein ABP19a-like	Cicer arietinum	28	569	21.9/20.7	6.95/6.91	XP_004513480.1
80	Low molecular weight phosphotyrosine protein phosphatase	Cicer arietinum	12	287	27.1/17.7	7.62/5.9	XP_004506147.1
	Other
44	Tropinone reductase homolog At5g06060-like	Cicer arietinum	27	250	28.5/22.5	6.43/6.66	XP_004492175.1
9	COBW domain-containing protein	Cicer arietinum	39	265	50.8/48.5	5.81/4.79	XP_004497313.1
15	6-phosphogluconate dehydrogenase, decarboxylating 3	Cicer arietinum	43	524	53.6/47.2	5.88/5.99	XP_004491970.2

^a Numbering corresponds to the 2-DE gel in Fig. 2a
^b Species name that the identified proteins is obtained via the MASCOT software from the NCBI database
^c Sequence coverage percentage of assigned peptides to the predicted protein
^d Score probability for the entire protein, obtained by Mascot search engine
^e TMr and EMr are theoretical molecular mass and experimental molecular mass, respectively
^f TpI and EpI are theoretical isoelectric point and experimental isoelectric point, respectively
^g Corresponding gene identification number in GenBank

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ISSN: 1471-2164

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