Fig. 3

Distribution of male-specific SNPs on LG22 and unplaced scaffolds NW_020523543.1 and NW_020523609.1. SNPs were counted using 100 kb sliding window with an output point every 500 bp and female- and male-specific SNPs were respectively indicated by red and blue color. a A large sex-determination region was identified on LG22, which is highlighted with a black box. The candidate sex-determining gene amh is located on this LG22, but not in the high density, male-specific SNP region. The region from 8 Mb to 10 Mb containing amh is zoomed in panel (b). c The National Center for Biotechnology Information (NCBI) Accession number NW_020523543.1 unplaced scaffold exhibits a region around 0.1 Mb harboring a small region (200 kb) with a high-density of male-specific SNPs. Meanwhile, sequence comparisons demonstrate that 7 male-biased RAD-tags (colored circles) on a total of 32 map with a high identity onto this scaffold. In contrast, few female-specific SNPs were enriched on this scaffold (red area). d The NCBI Accession number NW_020523609.1 unplaced scaffold is enriched in male-specific SNPs