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Fig. 6 | BMC Genomics

Fig. 6

From: Transcriptomic analyses of Aedes aegypti cultured cells and ex vivo midguts in response to an excess or deficiency of heme: a quest for transcriptionally-regulated heme transporters

Fig. 6

dsRNA knockdown in adult Aedes aegypti female midguts of candidate genes. a RNAi knockdown of the Ae. aegypti genes AAEL000417, AAEL000461, AAEL001938, AAEL002557, AAEL003318, AAEL004513, AAEL009531 and AAEL012440 in Ae. aegypti female midguts. b ZnMP fluorescence observed after RNAi knockdown of candidate heme transporter genes. Each colored point represents the average ZnMP fluorescence across a midgut normalized to an EGFP control. The colors of each point represent the specific experimental day that the point was observed; each experiment consisted of a sample size of ~n = 30 for each gene. ** Indicates genes that exhibited significantly different fluorescence than control with p-values below 0.01 and *** with p-values < 0.001. c ZnMP fluorescence of simultaneous RNAi knockdown of 4 genes, AAEL000417, AAEL003318, AAEL004513 and AAEL012440 in adult female mosquitoes. d AAEL003318 & AAEL012440 showed RT-qPCR confirmation of RNAi knockdown as they were distinct from the expression in cells targeted with dsEGFP (*** = p-val < 0.001). (e&f) Reproductive potential was measured through area measurements of ovaries at (e) 12 h and (f) 24 h post blood feeding. A slight decrease was observed at 12 h in the mix group (p-val = 0.0191) while no difference was observed at 24 h (p-val = 0.2723)

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BMC Genomics

ISSN: 1471-2164

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