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Table 1 Ae. aegypti cultured cell heme exposure experimental conditions and differential expression/cluster analysis results

From: Transcriptomic analyses of Aedes aegypti cultured cells and ex vivo midguts in response to an excess or deficiency of heme: a quest for transcriptionally-regulated heme transporters

Cell Type Incubation Time Type of Growth Media for Experimental Incubation Time Differential Expression Comparisons
[genes (genes w/TM domains)]
Cluster Analysis
[genes (genes w/TM domains)]
Normal Heme-Deficient Heme-Overexposure Normal vs Normal + 20 μM Heme Normal vs 0 μM Heme Normal vs 10 μM heme 0 μM vs 10 μM heme Import-like clusters Export-like clusters
Aag2 48 h Schneider’s Drosophila + FBS N/A Schneider’s Drosophila + FBS + 20 μM Heme 551 (211)     1611(455) 1391(385)
Aag2 72 h Schneider’s Drosophila + FBS Schneider’s Drosophila Schneider’s Drosophila +  10 μM Heme   587 (251) 1990 (737) 2274 (856) 745(238) 687(240)
Aag2 72 h Leibovitz’s L-15 + FBS Leibovitz’s L-15 Leibovitz’s L-15 +  10 μM Heme   4166 (1218) 4979 (1568) 3200 (1121) 905(229) 670(197)
A20 72 h Leibovitz’s L-15 + FBS SF900 II SF900 II + 10 μM Heme   745 (300) 2815 (658) 3692 (942) 1109(448) 757(143)