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Table 4 Polymorphisms between rye inbred lines in ScGAMYB sequence detected in nucleotide (nt) sequence, expected amino acid (aa) sequence and predicted protein second-structure (2D)

From: The GAMYB gene in rye: sequence, polymorphisms, map location, allele-specific markers, and relationship with α-amylase activity

changed nt position

type of nt change*

line with change

changed aa position

type of aa change

line with change

change in 2D

Lines: DS2, L35, M12, RXL10

 99

C → T

DS2

33

 129

G → A

DS2

43

 204

C → G

DS2

68

 226, 227

AA→TT

M12

76

N → F

M12

+

 259–261

ATC → GCG

M12

87

I → A

M12

+

 265

T → C

M12

89

Y → H

M12

+

 271

C → A

M12

91

 277

G → A

M12

93

D → N

M12

 564

C → T

DS2

188

Lines: 541, Ot1–3, DS2, L35, M12, RXL10

 681

C↔T

L35, M12, RXL10

227

 698

A↔C

L35, M12, RXL10

233

Q↔P

L35, M12, RXL10

+

 720

A↔G

L35, M12, RXL10

240

 735

G↔A

L35, M12, RXL10

245

 741

C↔T

L35, M12, RXL10

247

 944

G↔A

L35, M12, RXL10

315

S↔N

L35, M12, RXL10

1100

C → G

541, DS2

367

S → C

541, DS2

+

 1110

G → C

541, DS2

370

+

 1185

C → T

541, DS2

395

 1190, 1191

GG → GA/AG

541/DS2

397

R → K

DS2

+

 1375

T → C

541,Ot1–3

459

S → P

541, Ot1–3

  1. *The unidirectional arrows were used when the change concerned a minority of lines from the set of four or six lines tested. Changes important for the 2D protein structure and related to the effect of ScGAMYB on AMY are bolded