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Table 4 Polymorphisms between rye inbred lines in ScGAMYB sequence detected in nucleotide (nt) sequence, expected amino acid (aa) sequence and predicted protein second-structure (2D)

From: The GAMYB gene in rye: sequence, polymorphisms, map location, allele-specific markers, and relationship with α-amylase activity

changed nt position type of nt change* line with change changed aa position type of aa change line with change change in 2D
Lines: DS2, L35, M12, RXL10
 99 C → T DS2 33
 129 G → A DS2 43
 204 C → G DS2 68
 226, 227 AA→TT M12 76 N → F M12 +
 259–261 ATC → GCG M12 87 I → A M12 +
 265 T → C M12 89 Y → H M12 +
 271 C → A M12 91
 277 G → A M12 93 D → N M12
 564 C → T DS2 188
Lines: 541, Ot1–3, DS2, L35, M12, RXL10
 681 C↔T L35, M12, RXL10 227
 698 A↔C L35, M12, RXL10 233 Q↔P L35, M12, RXL10 +
 720 A↔G L35, M12, RXL10 240
 735 G↔A L35, M12, RXL10 245
 741 C↔T L35, M12, RXL10 247
 944 G↔A L35, M12, RXL10 315 S↔N L35, M12, RXL10
1100 C → G 541, DS2 367 S → C 541, DS2 +
 1110 G → C 541, DS2 370 +
 1185 C → T 541, DS2 395
 1190, 1191 GG → GA/AG 541/DS2 397 R → K DS2 +
 1375 T → C 541,Ot1–3 459 S → P 541, Ot1–3
  1. *The unidirectional arrows were used when the change concerned a minority of lines from the set of four or six lines tested. Changes important for the 2D protein structure and related to the effect of ScGAMYB on AMY are bolded