Fig. 2

Effects of heat-shock and cold-shock on spermathecal protein abundance. a Protein expression changes induced by cold-shock at 4 °C for 2 h (only significant proteins surviving 5% FDR threshold by the Benjamini-Hochberg method are shown). The two most significantly upregulated proteins are XP_026296654.1 (leucine-rich repeat transmembrane neuronal protein) and XP_395122.1 (probable tRNA N6-adenosine threonylcarbamoyltransferase). Each column represents the spermatheca from one queen. b GO term enrichment analysis. Grey triangles indicate the direction of expression driving the enrichment (relative to control). Only GO terms passing a 5% false discovery cut-off are shown (Benjamini-Hochberg method). MF stands for multifunctionality score (higher values are less multifunctional). Circle size is proportional to the number of proteins belonging to the GO term. c Protein expression changes induced by heat-shock at 40 °C for 2 h. d Enrichment analysis. e Temporal changes in expression of two previously identified candidate heat-shock biomarkers. Expression was significantly influenced by time and relative humidity (RH). Black bars indicate the mean