Fig. 5
From: The genetic basis of diurnal preference in Drosophila melanogaster
Expression of PER in clock neurons. Quantification of nuclear PER staining in N* (full lines) and D*(dashed lines) flies maintained in LD conditions. Shaded area represents dark. Representative staining (composite, Z-stacks) is shown below each panel (ZT13-ZT9, 4 h intervals). Points represent averages ± standard error. The appearance of the peak PER signal in ventral neurons (LNv: 5th-sLNv, sLNv, lLNv) was delayed in N* flies, as compared to D* flies (ZT1 vs ZT21–23), as indicated by significant time x genotype interactions: 5th-sLNv χ2 = 12,141, df = 11, p < 0.0001; sLNv χ2 = 4779.4, df = 11, p < 0.0001; lLNv χ2 = 7858, df = 11, p < 0.0001. The N* PER signal is stronger in sLNv (χ2 = 2416.6, df = 1, p < 0.0001), LNd (χ2 = 3924, df = 1, p < 0.0001) and DN1 (χ2 = 1799, df = 1, p < 0.0001) and weaker in DN2 (χ2 = 523.2, df = 1, p < 0.05)