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Fig. 1 | BMC Genomics

Fig. 1

From: Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1

Fig. 1

OsHV-1 RNA and DNA levels in the gill tissue of 15 OsHV-1-positive oysters (C. gigas, S1-S15) sampled in the Goro lagoon, Italy. a. Quantification of OsHV-1 transcription by RT-qPCR (bars, left axis) and OsHV-1 DNA loads (black diamonds, right axis). The OsHV-1 ORF104 transcript levels normalized to the expression of the C. gigas housekeeping gene elongation factor 1-apha, was considered as a proxy of the viral transcriptional activity. OsHV-1 DNA content was measured as DNA copy number per μl. Grey bars indicate the six samples selected for sncRNA and mRNA HT-sequencing. In these six samples, red dots represent the number of RNA reads mapping to the OsHV-1 genome. The samples denoted by grey bars were selected for RT-qPCR analysis. b. Subdivision of samples S1-S6 based on the ratio of OsHV-1 RNA over DNA (δ value) grouping pairs of samples into low, mid, or high δ samples

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BMC Genomics

ISSN: 1471-2164

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