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Table 2 Gene Ontology (GO) terms enriched and KEGG pathways upregulated relating to aerobic or anaerobic metabolism and calcium cycling in each tissue. For full list and p values, see Table S2. None means no GO term was enriched and no KEGG pathways upregulated

From: Skeletal muscle and cardiac transcriptomics of a regionally endothermic fish, the Pacific bluefin tuna, Thunnus orientalis

Tissue Compared against Metabolic or calcium cycling upregulated GO terms
Pathway GO terms and KEGG pathways upregulated for each category
White muscle Red muscle Anaerobic metabolism Molecular Function: 1 (6-phosphofructokinase activity)
Biological Process: 6 (Glycogen metabolic process, Glycolytic process, Fructose 6-phosphate metabolic process, Gluconeogenesis, Carbohydrate metabolic process, Glycolytic process through fructose-6-phosphate)
KEGG Pathway: 1 (Glycolysis/ gluconeogenesis)
Calcium cycling Cellular Component: 1 (Endoplasmic reticulum membrane)
Biological Process: 2 (Release of sequestered calcium ion into cytosol, Calcium ion homeostasis)
KEGG Pathway: 1 (Calcium signalling pathway)
Red muscle White muscle Aerobic metabolism Cellular Component: 3 (Mitochondrial matrix, Proton-transporting ATP synthase complex, coupling factor f(o), Mitochondrial membrane)
Molecular Function: 4 (CoA-ligase activity, Cytochrome-c oxidase activity, NAD binding, Isocitrate dehydrogenase activity)
Biological Process: 9 (Tricarboxylic acid cycle, ATP synthesis coupled proton transport, ATP hydrolysis coupled cation transmembrane transport, Mitochondrial ATP synthesis coupled electron transport, Electron transport chain, Acyl-CoA metabolic process, Acetyl-CoA biosynthetic process, ATP synthesis coupled electron transport, Mitochondrial transport)
KEGG Pathway: 2 (TCA cycle, Oxidative phosphorylation)
Calcium cycling Molecular Function: 1 (Calcium-transporting ATPase activity)
Deep white muscle Superficial white muscle None None
Superficial white muscle Deep white muscle Calcium cycling Molecular Function: 1 (Calcium-release channel activity)
Deep red muscle Superficial red muscle 0 None
Superficial red muscle Deep red muscle Calcium cycling Molecular Function: 1 (Calcium-release channel activity)
KEGG Pathway: 1 (Calcium signalling pathway)
Atrium Ventricle Anaerobic metabolism Molecular Function: 1 (6-phosphofructokinase activity)
Biological Process: 2 (Glycolytic process through fructose-6-phosphate, Fructose 6-phosphate metabolic process)
Calcium cycling Molecular Function: 2 (Calcium ion binding, Calcium-transporting ATPase activity)
Biological Process: 2 (Calcium ion transmembrane transport, Regulation of calcium ion transmembrane transporter activity)
Ventricle Atrium Aerobic metabolism Cellular Component: 4 (Respiratory chain complex, Inner mitochondrial membrane protein complex, Mitochondrial respiratory chain, Mitochondrial membrane)
Molecular Function: 4 (Proton-transporting ATP synthase activity, rotational mechanism, Electron transfer activity, CoA-ligase activity, Acid-thiol ligase activity)
Biological Process: 2 (Fatty acid beta-oxidation, ATP synthesis coupled proton transport)
KEGG Pathway: (TCA cycle, oxidative phosphorylation)
Anaerobic metabolism (3) Biological Process: 2 (Carbohydrate metabolic process, Glycolytic process)
KEGG Pathway: 1 (Glycolysis / gluconeogenesis)
Calcium cycling (1) Molecular function: 1 (Ryanodine-sensitive calcium-release channel activity)
Spongy ventricle Compact ventricle 0 None
Compact ventricle Spongy ventricle 0 None