Fig. 5

cbp-1 is required for AFT. a A model of the cbp-1 gene indicating exon/intron structure and the location and extent of the deletion alleles used in this study. b Normalized cbp-1 expression in our RNA sequencing samples. c-d Animals were tested on 0 mM and 400 mM exogenous ethanol. Relative speeds were calculated as treated over untreated speeds (left Y-axes). AFT is quantified as the recovery of speed between 10 and 30 min of exposure (right Y-axes). All genotypes on the same graph were tested simultaneously on the same plates. Wild-type (N2) animals develop AFT; swsn-9(ok1354) mutant animals do not develop AFT. c Heterozygous cbp-1(ok1491) mutant animals over either a balancer (bal) or wild type (+) chromosome failed to develop AFT. d Heterozygous cbp-1(bm2) mutant animals maintained over a balancer (bal) chromosome failed to develop AFT, while heterozygous cbp-1(bm2) over a wild type chromosome did develop AFT; the recovery of speed was statistically different from wild-type for cbp-1(bm2)/balancer. Error bars indicate S.E.M. Paired one-tailed Student’s t tests were used for statistical comparisons of speeds at 10 and 30 min; One-way ANOVA, with Tukey’s multiple comparison test, was used to compare the initial sensitivity and recovery of speed across the strains. For indicated comparisons: ns, not significant; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001