Fig. 2

Taxonomic distribution of the novel flag-3, flag-4 and flag-5 loci among the Enterobacterales. The phylogeny was constructed as previously described [15] on the basis of four house-keeping proteins, GyrB, InfB, RecA and RpoB. The trimmed concatenated alignment comprised of 2613 amino acid sites and the tree was constructed using the best-fit evolutionary model JTTDCMut+I + G4. Bootstrap support (n = 1000 replicates) values > 50% are indicated for the major clades. The presence of the different loci are indicated by different coloured shapes, blue triangles (flag-3a), red diamonds (flag-3b), green circles (flag-4) and purple triangles (flag-5), while those taxa where both flag-2 and flag-3 loci are present are indicated by pink squares. While the prevalence of flag-1 loci is not shown, those taxa that incorporate flag-2 loci are indicated by green-coloured branches