RNA-Seq and differential gene expression analysis in Temora stylifera copepod females with contrasting non-feeding nauplii survival rates: an environmental transcriptomics study

Table 3 List of unigenes and isoforms tested for Temora stylifera transcriptome validation

Name	A_L (bp)	log₂-FC	p-adj		Primers	%E
A5¹	130	−4.61	9⁻¹²	F	GCCTGTTGCCGGAAACTTTT	110
A5¹	130	−4.61	9⁻¹²	R	TTCTGGGCCGTCATTGACTC	110
CREBL¹	111	−2.04	2⁻³	F	GTACAAGCTGGAGAGGAGTCG	103
CREBL¹	111	−2.04	2⁻³	R	GCCTTATTTGCCCTCTCCCT	103
me31b¹	136	5.42	4⁻³	F	TTCTGGACGAAGCGGACAAG	114
me31b¹	136	5.42	4⁻³	R	CGCATGAAGGACTCGACTGT	114
Ppa2¹	140	−3.72	1⁻³	F	GCTTTGCCTTAAACTGCGCT	113
Ppa2¹	140	−3.72	1⁻³	R	CGGCAGGTAGTCAAACAGGT	113
Obst-E¹	228	−1.62	9⁻⁵	F	CAAGATCGACTGTCTGGGCA	103
Obst-E¹	228	−1.62	9⁻⁵	R	CGAGCCTTTCCACTCCACTT	103
ARIH1¹	200	−1.76	2⁻³	F	AGATGTGGGGCTGCAACTAC	106
ARIH1¹	200	−1.76	2⁻³	R	CTCAATCTTCTCCAGCGGCA	106
ARSB¹	116	−1.29	4⁻³	F	AACAACAGGGGCTTCAACCA	108
ARSB¹	116	−1.29	4⁻³	R	TCAAACTCTGGCACCCTGTC	108
Hsp70¹	133	−1.25	3⁻²	F	CCATTCAGGTCTACGAGGGC	102
Hsp70¹	133	−1.25	3⁻²	R	TTGGCGTCAATGTCGAAGGT	102
VLDL1²	132	1.44	4⁻²	F	ATCGCAGGGTCATTGTCCAG	112
VLDL1²	132	1.44	4⁻²	R	TGCGTATGTCTCGACCAGTG	112
MOB1B²	156	−9.48	4⁻¹⁰	F	TTGTCCTGTCATGTCGGCAG	89
MOB1B²	156	−9.48	4⁻¹⁰	R	TTGCTGGGGAACAAGGACTC	89
Vasa²	138	7.14	3^− 4	F	CGCCTTCAACGATCTCCAGT	103
Vasa²	138	7.14	3^− 4	R	GCCGAGAACATAAGGGTGGT	103
PAFAH²	163	6.93	2⁻³	F	GCCTTCACCTCGCTCTTCAG	87
PAFAH²	163	6.93	2⁻³	R	AGGCGTATCGATTGCAACCT	87

Name, amplicon length (A_L) (bp), log₂-Fold-Change (log₂-FC), adjusted p-value (p-adj), primer sequence and amplification efficiency percentage (%E) are shown. ‘¹’: unigenes; ‘²’: isoforms

ISSN: 1471-2164