Skip to main content

Table 1 Detected candidate proteins interacting with SPL13 by IPMS analysis

From: Transcriptome-IPMS analysis reveals a tissue-dependent miR156/SPL13 regulatory mechanism in alfalfa drought tolerance

Proteins Reported roles and description Sequence coverage (%) # Peptides # Spectra Molecular weight (KDa)
50S ribosomal protein L22 Early stages of 50S ribosomal protein assembly 18 4 7 21.88
60s ribosomal protein L11 Biogenesis of 60S ribosomal subunits 49.72 6 11 20.67
Arginine biosynthesis bifunctional protein Involved in the cyclic version of arginine biosynthesis 26.72 7 21 48.6
Beta-xylosidase/alpha-L-arabinofuranosidase Releases xylose and arabinose from cell walls 25.32 15 30 83.67
Cafeic acid 3-O-methlyltransferase (COMT1) Catalyzes the conversion of caffeic acid to ferulic acid and of 5-hydroxyferulic acid to sinapic acid. 17.81 6 31 39.92
Cell division control protein 2 homolog 1 control of the eukaryotic cell cycle 17.87 4 11 33.46
Chalcone synthase 1 naringenin-chalcone or chalcone biosynthesis 15.17 5 14 42.74
Chalcone synthase 2 31.62 9 38 42.68
Chalcone synthase 4 28.53 8 34 42.63
Cyclic beta-(1,2)-glucan synthase biosynthesis of cyclic beta-(1,2)-glucan 15.11 29 86 315.55
Dihydroflavonol 4-reductase anthocyanin biosynthesis 33.18 6 12 24.37
Eukaryotic translation initiation factor 5A-1 mRNA-binding protein involved in translation elongation 32.3 3 32 17.65
Glutamate synthasee [NADH] amyloplastic assimilation of symbiotically fixed nitrogen into amino acids 19.51 30 99 240.22
Glutamine synthetase cytosolic isozyme homeostatic control of glutamine synthesis 16.85 4 9 39.08
Glutamine synthetase leaf isozyme, chloroplastic Re-assimilation of the ammonia generated by photorespiration 14.95 6 15 47.09
Glycogen synthase kinase-3 homolog − 1 Brassinosteroids signaling and abiotic stress response 21.41 6 11 46.74
Glycogen synthase kinase-3 homolog − 2 25.3 7 94 46.03
Glycogen synthase kinase-3 homolog − 3 37.23 11 27 46.24
Isoflavone reductase, IFR Reduces achiral isoflavones to chiral isoflavanones 13.52 4 5 35.43
Long-distance movement protein facilitate the cell-to-cell movement 17.93 3 3 26.67
Malate dehydrogenase, chloroplastic Catalyzes a reversible NAD-dependent dehydrogenase reaction involved in central metabolism 16.87 3 3 35.52
Methylthioribose-1-phosphate isomerase Catalyzes methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P) interconversion 38.14 11 15 42.24
Mitogen-activated protein kinase homolog mitogenic induction of symbiotic root nodules 21.19 6 32 44.37
Nodulation protein D1 Regulates other nodulation proteins 15.2 3 3 34.86
Nodulation protein E synthesize NOD factor fatty acyl chain 26.68 7 9 41.64
Nodulin-25 development and function of nodules 19.51 3 6 27.51
Phenylalanine ammonia-lyase catalyzes the deamination of phenylalanine to cinnamate and ammonia 21.38 12 21 78.82
Phosphoenolpyruvate carboxylase catalyzes the addition of bicarbonate to phosphoenolpyruvate 17.08 11 16 110.82
Polygalacturonase depolymerization of pectates 15.44 6 29 43.93
Replication protein 1A stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. 33.13 25 170 111.95
Ribulose bisphosphate carboxylase large chain Catalyzes the carboxylation of D-ribulose 1,5-bisphosphate and oxidative fragmentation of the pentose substrate 24.05 12 21 52.59
Ribulose bisphosphate carboxylase small chain Catalyzes the carboxylation of D-ribulose 1,5-bisphosphate and oxidative fragmentation of the pentose substrate 46.67 7 34 20.24
RNA-directed RNA polymerase catalyses synthesis of the RNA strand complementary to a given RNA template 15.14 9 34 93.6
RNA-directed RNA polymerase 2a required for cytoplasmic and chromatin RNA silencing pathways 16.91 9 13 93.46
Serine/threonin-protein phosphatase PP1 essential for cell division and glycogen metabolism by dephosphorylating substrates 29.6 8 97 36.23
Serine/threonin-protein phosphatase PP2A Dephosphorylates and activates the actin-depolymerizing factor ADF1 18.85 3 5 35.69
Sucrose synthase Sucrose-cleaving enzyme that provides UDP-glucose and fructose 15.65 8 31 92.27
Tyrosine recombinase XerC Catalyze the cutting and rejoining of the recombining DNA molecules 15.72 3 3 33.75
Vacuolar membrane protease vacuolar sorting and osmoregulation 21.96 14 45 112.35
Vacuolar protein sorting/targeting protein 10 sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins 18.45 22 67 161.33
  1. Candidate proteins were considered when at least three peptides with six spectra were present from the protein while undergoing the database search as specified in the method part of the article. Thermo Q-Exactive Orbitrap mass spectrometer coupled to an Easy-nLC 1000 nano LC system were used to analyze samples