| |
Group
|
Classification
|
N
|
Placental viral load
|
Serum viral load
|
Thymus viral load
|
Fetal weight (g)
|
Crown rump length (cm)
|
Brain/ liver ratio
|
|---|
|
Placenta
|
CTRL
|
NA
|
15
|
ND
|
0 (0)
|
ND
|
757.7 (160.9)
|
27.2 (2.6)
|
1.4 (0.3)
|
|
UNINF
|
R
|
16
|
0 (0)
|
0 (0)
|
0 (0)
|
753.2 (251.4)
|
26.8 (3.6)
|
1.3 (0.4)
|
|
PLCO-VIA
|
R
|
12
|
5.3 (1.5)
|
0 (0)
|
0 (0)
|
751.5 (292.0)
|
26.8 (3.8)
|
1.5 (0.5)
|
|
PLCO-MEC
|
R
|
4
|
5.6 (2.7)
|
0 (0)
|
0 (0)
|
868.2 (228.6)
|
28.8 (2.7)
|
1.3 (0.4)
|
|
LVL-VIA
|
T
|
7
|
6.3 (1.45)
|
6.1 (1.1)
|
3.1 (0.5)
|
865.9 (200.1)
|
28.4 (3.1)
|
1.2 (0.3)
|
|
LVL-MEC
|
S
|
6
|
7.4 (0.9)
|
7.1 (0.9)
|
3.1 (0.9)
|
798.0 (145.8)
|
27.4 (2.8)
|
1.4 (0.6)
|
|
HVL-VIA
|
T
|
14
|
7.1 (1.1)
|
7.2 (0.8)
|
5.5 (0.6)
|
801.8 (257.8)
|
27.4 (2.8)
|
1.3 (0.4)
|
|
HVL-MEC
|
S
|
12
|
7.3 (0.6)
|
8.2 (0.8)
|
6.1 (0.6)
|
732.0 (143.3)
|
25.5 (1.6)
|
0.8 (0.3)
|
|
Thymus
|
CTRL
|
NA
|
14
|
ND
|
0 (0)
|
ND
|
748.8 (163.9)
|
27.1 (2.7)
|
1.4 (0.3)
|
|
UNINF
|
R
|
15
|
0 (0)
|
0 (0)
|
0 (0)
|
752.5 (260.2)
|
26.8 (3.7)
|
1.3 (0.5)
|
|
PLCO-VIA
|
R
|
15
|
5.4 (1.5)
|
0 (0.01)
|
0 (0)
|
734.1 (274.6)
|
26.7 (3.6)
|
1.5 (0.4)
|
|
PLCO-MEC
|
R
|
5
|
5.4 (2.4)
|
0 (0)
|
0 (0)
|
894.0 (206.2)
|
29.0 (2.4)
|
1.2 (0.4)
|
|
LVL-VIA
|
T
|
8
|
6.3 (1.4)
|
6.4 (1.2)
|
3.1 (0.5)
|
839.6 (199.7)
|
28.1 (3.0)
|
1.3 (0.3)
|
|
LVL-MEC
|
S
|
4
|
7.1 (1.0)
|
7.1 (1.1)
|
3.2 (0.9)
|
795.0 (173.4)
|
27.2 (2.8)
|
1.5 (0.8)
|
|
HVL-VIA
|
T
|
15
|
7.2 (1.2)
|
7.2 (0.8)
|
5.6 (0.6)
|
789.1 (253.2)
|
27.2 (2.8)
|
1.4 (0.4)
|
|
HVL-MEC
|
S
|
14
|
7.2 (0.6)
|
8.1 (0.8)
|
6.0 (0.7)
|
748.5 (127.3)
|
26.1 (1.7)
|
0.9 (0.3)
|
- Fetal phenotypes for groups based on PRRSV viral load as determined by quantitative PCR on fetal placenta, serum and thymus, in the form of group average in log10 copies/μL. Numbers for phenotypic measurements are displayed as the mean and standard error in parentheses. Further subdivision of fetuses was made based on preservation status at the time of sample collection with only fetuses classified as either viable (VIA) and meconium stained (MEC) used in the present work. Group control (CTRL) was mock infected, gilt infected but no virus detected in the fetus (UNINF), virus detected in the placenta only with viable (PLCO-VIA) or meconium-stained fetus (PLCO-MEC), low viral load in the fetus with viable (LVL-VIA) or meconium-stained fetus (LVL-MEC), and high viral load in the fetus with viable (HVL-VIA) or meconium-stained fetus (HVL-MEC). Classifications; NA (not applicable as not challenged), resilient (R), tolerant (T), susceptible (S)
