Fig. 4

Phylogenetic distribution of RNA-dependent RNA polymerase (RdRP) proteins in Acari species: Varroa destructor (Vd), V. jacobsoni (Vj), Tropilaelaps mercedesae (Tm), Metaseiulus occidentalis (Mo), Ixodes scapularis (Is), Dermatophagoides pteronyssinus (Dp), Euroglyphus maynei (Em), Tetranychus urticae (Tu), Sarcoptes scabiei (Sc) and Psoroptes ovis (Po). The nematode species, Caenorhabditis elegans (Ce) was used as outgroup. The species names were abbreviated for convenience and the numbers (1, 2, 3, 4 and 5) following their names indicate the protein copies found in each species. The tree was constructed using PhyML [43], with the model recommended by Lefort et al. [44] under the Akaike information criterion (AIC) (LG + G + I + F) with 500 bootstrap replicates and is based on the amino acid alignment of the conserved RdRP domain (IPR007855/PF05183) using MAFFT [42]