Fig. 4

The knockdown of h-jou by si-RNA decreases the proliferation. a Cells number of HCT116 transiently transfected cells with LNA-siRNA directed against jouvence compared to the control non-transfected cells (Co = treated with the same amount of RNAiMAX), and to the control (Si-Co = transfected cells with a siRNA-control without target), determined by VicellXR 72 h post-seeding. b The expression level (Fold change) of the siRNA transfected HCT116 cells compared to the non-transfected cells, determined 72 h post-seeding (same batch of cells than A). c Cells number of MCF7 transiently transfected cells compared to their two respective controls, as in A, determined by VicellXR. d Same batch of cells than C, but spread and growth in 96 cell-plates and evaluated by luminescence (CellTiter-Glo) after 96 h. e Cells number of U87 transiently transfected cells with LNA-siRNA compared to their respective controls, determined by VicellXR. f Same batch of cells than E, but spread and growth in 96 cell-plates and evaluated by luminescence after 96 h. g Cells number of A549 transiently transfected cells with LNA-siRNA compared to their control, determined by VicellXR. h Same batch of cells than G, but spread and growth in 96 cell-plates and evaluated by luminescence after 96 h. i Cells number of HUVEC (primary cells) transiently transfected cells with LNA-siRNA compared to their respective control, determined by VicellXR. j Same batch of cells than I, but spread and growth in 96 cell-plates and evaluated by luminescence. Statistics: For the VicelXR (A-C-E-G-I) n = 3, for the Luminescence CellTiter-Glo (B-D: n = 27), (H: n = 8), (J: n = 10). For each figure, two independent experiments have been performed. (p-values: * p < 0,05; ** p < 0,005; *** p < 0,0005). Errors bars represent the mean +/− S.E.M. (p-value were calculated using the one-tail unpaired t-test using GraphPad Prism)