Table 1 Short list of the main genes deregulated in overexpressing jouvence HCT116 cells revealed by RNA-Seq

Gene_id

Fold Change

padj

Gene Name

Description

 ENSG00000124766

2,82

1,86E-273

SOX4

SRY (sex determ region Y)-box 4

 ENSG00000128564

3,43

1,93E-264

VGF

VGF nerve growth factor inducible

 ENSG00000049130

2,54

2,33E-219

KITLG

KIT ligand

 ENSG00000175745

6,80

1,64E-203

NR2F1

nuclear receptor subfamily 2 group F

 ENSG00000158710

2,47

6,78E-200

TAGLN2

transgelin 2

 ENSG00000089472

9,35

6,17E-197

HEPH

hephaestin

 ENSG00000077274

10,32

7,35E-192

CAPN6

calpain 6

 ENSG00000079215

5,15

2,63E-159

SLC1A3

solute carrier family 1 (glial affinity)

 ENSG00000127528

2,46

7,89E-150

KLF2

Kruppel-like factor 2 (lung)

 ENSG00000146376

2,89

3,19E-148

ARHGAP18

Rho GTPase activating protein 18

 ENSG00000165949

6,46

9,82E-145

IFI27

interferon, alpha-inducible protein 27

 ENSG00000138434

1,98

1,37E-117

SSFA2

sperm specific antigen 2

 ENSG00000120738

2,61

3,23E-115

EGR1

early growth response 1

 ENSG00000204574

2,24

3,36E-110

ABCF1

ATP-binding cassette, sub-family F

 ENSG00000091136

2,06

4,04E-105

LAMB1

laminin, beta 1

 ENSG00000179094

2,25

5,37E-105

PER1

period circadian clock 1

 ENSG00000140450

2,13

8,38E-105

ARRDC4

arrestin domain containing 4

 ENSG00000115457

2,53

1,12E-101

IGFBP2

insulin-like growth factor bind. prot2

 ENSG00000105373

1,94

4,02E-98

GLTSCR2

glioma tumor suppressor

 ENSG00000267761

0,03

1,91E-269

CTD-2130O13.1

–

 ENSG00000142910

0,32

2,10E-259

TINAGL1

tubulointerstitial nephritis antigen-like

 ENSG00000101188

0,35

2,04E-238

NTSR1

neurotensin receptor 1 (high affinity)

 ENSG00000130508

0,18

1,08E-230

PXDN

peroxidasin homolog (Drosophila)

 ENSG00000169035

0,15

6,81E-222

KLK7

kallikrein-related peptidase 7

 ENSG00000099994

0,14

6,04E-219

SUSD2

sushi domain containing 2

 ENSG00000175906

0,25

7,80E-206

ARL4D

ADP-ribosylation factor-like 4D

 ENSG00000135074

0,37

1,79E-178

ADAM19

ADAM metallopeptidase domain 19

 ENSG00000103044

0,23

9,15E-170

HAS3

hyaluronan synthase 3

 ENSG00000108244

0,07

9,31E-170

KRT23

keratin 23 (histone deacet. inducible)

 ENSG00000124762

0,49

1,36E-165

CDKN1A

cyclin-dependent kinase inhibitor 1A

 ENSG00000101311

0,30

1,40E-164

FERMT1

fermitin family member 1

 ENSG00000089356

0,16

1,72E-150

FXYD3

FXYD domain cont. Ion transport

 ENSG00000197081

0,36

4,84E-148

IGF2R

insulin-like growth factor 2 receptor

 ENSG00000167779

0,36

1,08E-145

IGFBP6

insulin-like growth factor bind. prot6

 ENSG00000180921

0,43

2,15E-131

FAM83H

family with sequence similarity 83

 ENSG00000117394

0,49

6,20E-124

SLC2A1

solute carrier family 2

 ENSG00000148346

0,31

1,58E-122

LCN2

lipocalin 2

 ENSG00000130513

0,51

5,91E-121

GDF15

growth differentiation factor 15

#Term (KEGG pathway)

ID

Input number

Background number

P-Value-corr

Ribosome

hsa03010

89

138

3,06E-42

Metabolic pathways

hsa01100

189

1243

8,03E-17

Pathways in cancer

hsa05200

75

397

4,62E-10

Endocytosis

hsa04144

57

260

8,96E-10

Spliceosome

hsa03040

36

134

4,40E-08

Adherens junction

hsa04520

26

74

8,45E-08

HTLV-I infection

hsa05166

51

259

1,47E-07

MAPK signaling pathway

hsa04010

50

255

2,06E-07

Herpes simplex infection

hsa05168

41

186

2,50E-07

RNA transport

hsa03013

38

172

7,33E-07

Epstein-Barr virus infection

hsa05169

42

204

7,33E-07

Tight junction

hsa04530

32

139

3,40E-06

Ras signaling pathway

hsa04014

42

228

8,83E-06

Wnt signaling pathway

hsa04310

31

143

1,39E-05

Huntington’s disease

hsa05016

37

193

1,60E-05

Proteoglycans in cancer

hsa05205

38

205

2,19E-05

Ribosome biogenesis in eukaryotes

hsa03008

23

89

2,50E-05

Protein processing in endoplasmic reticulum

hsa04141

33

166

2,57E-05

Axon guidance

hsa04360

34

176

2,97E-05