Fig. 8

Edited CGIs affected by HDR show statistically significant p-values. In evaluating the methylation variance between CRISPR-edited and control mouse epigenomes, top CGIs for each comparison were determined by ranking of p-values observed and removing those CGIs that did not display greater than 50% of observed CpGs with methylation increases above 20%. Those top CGIs containing HDR edits had more significant p-values for methylation increase above biological variance (20% change) as compared to other CGIs across the mouse genome for HDR1 Animal (a) and HDR2 Animal (b). A CGI with a complete modification of its methylome displayed a significant increase of p-value over the control (a), while a CGI with only a partial modification displayed an increase of p-value over the control at a lesser significance (b). The top CGIs with methylation change within NHEJ1 Animal containing a NHEJ edit had no significant increase of p-value above biological variance, and the edited CGI was not among the top CGIs with methylation change (c). Comparison of CGIs in unedited Control 2 Animals displayed a low significance of change for the top CGIs (d)