Detection of CRISPR-mediated genome modifications through altered methylation patterns of CpG islands

BMC Genomics

Table 3 Case 1 p-value rankings for HDR1 Animal compared to Control 1 Animal

					Degree of Experimental Methylation Level Higher Than Control
Chromosome	CpG Island Start Location	CpG Island End Location	p-value	CpG Count	1–20% Proportion	1–20%	21–40%	41–60%	61–80%	81–100%
15	11,035,894	11,038,084	3.852E-48	91	0.034	3	9	26	45	6
5	141,975,051	141,975,377	6.732E-12	23	0.043	1	10	10	2	0
15	89,523,499	89,524,359	1.555E-09	62	0.388	19	21	8	1	0
1	126,092,971	126,093,200	2.973E-09	16	0.125	2	5	8	1	0
7	45,098,344	45,098,676	2.978E-09	15	0.000	0	0	4	7	3
10	128,262,409	128,262,692	2.055E-08	12	0.083	1	10	1	0	0
8	116,827,244	116,827,574	2.268E-08	13	0.077	1	1	9	2	0
4	22,057,643	22,057,924	6.841E-08	11	0.000	0	10	1	0	0
15	38,118,394	38,118,655	7.345E-08	16	0.250	4	11	1	0	0
19	38,914,796	38,915,109	9.360E-08	12	0.083	1	7	4	0	0

The p-value represents the upper-tail p-value for the hypothesis test of higher methylation in the experimental mouse than the control mouse. The CG count includes the total number of CGs in the CpG island. The proportion and category counts only refer to the number of CGs where the experimental CG methylation level is higher than the control CG methylation level

ISSN: 1471-2164