Fig. 2

Influence of vitrification on the gene expression in cat ovarian cortex. a Volcano plot showing differentially expressed genes (DEGs) meeting the conditions of |fold change| ≥ 2 and q-value < 0.05. Yellow dots represent genes up-regulated in vitrification (V) vs. fresh tissues (F), blue dots represent genes down-regulated in V vs. F, and grey dots represent the genes that were not differentially expressed in V vs. F. b Heatmap of the one-way Hierarchical Clustering Analysis (Euclidean Method, Complete Linkage) using Z-score for normalized value (log₂ based) for comparison pair V vs. F. Bottom of heatmap is labeled with sample IDs representing condition (V, F) and animal (1 to 6)