Fig. 1From: Deep sampling and pooled amplicon sequencing reveals hidden genic variation in heterogeneous rye accessionsScatter plot of Variant Allele Frequency (VAF) data from GATK HaplotypeCaller. VAF is plotted on the x-axis. Black dots represent every predicted variant. The number of accessions predicted to harbor the variant is plotted on the y-axis. Data is plotted on the z-axis to separate different variants that share the same VAF and number of accessions. The percentage of the total data from VAF 0 to a specific frequency is overlaid in red. For example, 75% of all predicted nucleotide variants have a VAF of 0.05 or lowerBack to article page