Skip to main content

Table 3 Relative abundance (%) of ASVs observed in PCR water samples in setups 2 and 3

From: Exploring protocol bias in airway microbiome studies: one versus two PCR steps and 16S rRNA gene region V3 V4 versus V4

ASV Lowest Classification Setup 2 Setup 3
06f825b512d903b9230e1a55d87359ee f__Streptococcaceae; g__Streptococcus 35.39 20.77
ddfd49f939f92958b1ec816741055348 f__Oxalobacteraceae; g__Ralstonia; s__ 12.36 0.00
394eda29c886632f514dd94b58381186 f__Pasteurellaceae 8.99 0.00
d32e579b3ae7b2aae8d5bf9f027c29af f__Comamonadaceae 8.99 0.00
5648dccee530d68ceb3e4d7d22cf8756 f__Pseudomonadaceae; g__Pseudomonas 7.87 0.00
4f5efd25dacb5d639316e7291ff6ff8b f__Neisseriaceae; g__Neisseria 7.87 7.69
85c44c83eddc5d3028261a1000b7d0e1 f__Gemellaceae 5.62 0.00
923f521b9cf313f1f95c9367e09bbc1c f__Veillonellaceae; g__Veillonella; s__dispar 5.62 12.31
dcba105f35d8ebc9e22269c7491ad3a7 f__Xanthomonadaceae; g__Stenotrophomonas; s__geniculata 5.06 0.00
df8456a1abbfb4c8a2c450b44378d4cb f__Actinomycetaceae; g__Actinomyces; s__ 2.25 0.00
d46e2205f0c6ecf67b51f83d111c509c* f__Enterobacteriaceae 0.00 35.38
edc9e5c16e40aff1eadce6597940f08f f__Streptococcaceae; g__Streptococcus; s__ 0.00 13.85
65d43491988bfe557da4d86a5ba25dae f__Staphylococcaceae; g__Staphylococcus 0.00 10.00
  1. *Escherichia ASV also observed to differentiate mock community samples and NCS in setups 2 and 3. Bioinformatic processing steps were performed up until the removal of contaminants identified using Decontam. Taxonomic rank is described using prefixes (f__: family, g__: genus, s__: species)