Fig. 7
From: New insights into the evolution and functional divergence of the CIPK gene family in Saccharum
Transient overexpression of ScCIPKs in Nicotiana benthamiana leaves. A RT-PCR analysis of ScCIPKs in the N. benthamiana leaves 2 days after infiltration with Agrobacterium strain GV3101 that carried the vector 35S::00 or 35S::ScCIPKs (The gels were selected from the same gel with the same exposure, and the unmodified figure was shown in the Supplementary Fig. S4-S7). B The transcripts of the immunity-associated marker genes in 35S::ScCIPKs -transiently expressing leaves at 2 days after infiltration. NtEF1-α was used for normalization of the transcript levels. Mock, the Agrobacterium strain carrying 35S::00. All of the data points were expressed as the mean ± SE (n = 3). Different lowercase letters indicate a significant difference between 35S::00 and 35S::ScCIPKs in each gene, as determined by the Duncan’s new multiple range test (p-value < 0.05)