Fig. 3

Overrepresented protein classes and pathways in the occluded middle cerebral artery (MCA) from female rats. a. Seventeen PANTHER protein classes and 18 PANTHER and Reactome pathways were overrepresented amongst the differentially expressed genes in the occluded MCAs from female rats compared with control MCAs. In the non-occluded MCAs, only 5 overrepresented pathways and protein classes were identified all of which were also identified for the occluded MCAs. b. Scatterplot illustrating the differentially expressed genes annotated to the overrepresented PANTHER protein class, intercellular signal molecule, with fold change on the x-axis and -log10(p-value) on the y-axis (highlighted if fold change ≥4 or -log10(p-value) ≥ 5.5). Chemokine (C-C motif) ligand 2 (Ccl2) had the highest fold change within this protein class. c. Scatterplot illustrating the differentially expressed genes annotated to the overrepresented Reactome pathway, cytokine signaling in immune system, with fold change on the x-axis and -log10(p-value) on the y-axis (highlighted if fold change ≥4 or -log10(p-value) ≥ 5.5). Suppressor of cytokine signaling 3 (Socs3) had the highest fold change and second lowest p-value within this pathway