Fig. 5
From: Environmental RNAi pathways in the two-spotted spider mite
Fate of synthetic short hairpin RNAs fed to spider mites. a Actin targeted structured short hairpin RNAs fed to animals via soaking. Designs mimic modes of miRNA biogenesis: Dicer processed (shRNA), Ago processed short loop RNAs (SL1, SL2), and loop derived RNAs (Loop). Red sequence indicates portion targeted to Actin. Yellow highlight show G/C “clamp”. b Percent expression of target gene (Actin) following ingestion of long double-stranded RNA (dsRNA) or short RNAs (a) determine by qRT-PCR and normalized to rRNA. Error bars are standard deviation. c-d Alignment of RNAs sequenced from animals fed SL2 (c) or shRNA (d) to the synthetic RNA sequences. Red sequence shows portion targeted to Actin. e-f Absolute number of reads mapping to short hairpin RNAs SL2 (e) and shRNA (f)