Fig. 5

The F. hepatica life cycle stages have distinct secretome profiles. a Graphical representation of the top 50 proteins secreted by the immature flukes representing 87% of the total protein secreted (based on emPAI values of three biological replicates), highlight that the immature fluke secretome is predominantly comprised of cathepsin peptidases and cathepsin peptidase inhibitors. b Graphical representation of the composition of the F. hepatica stage-specific secretomes, based on the emPAI abundance. Relative abundance is shown by a blue to red scale, depicting low to high levels of abundance, respectively. c Venn diagram representing the comparative analyses of proteins secreted by newly excysted juveniles 24 h post excystment (NEJ 24 h), the immature parasites (Juv21dpi) and the adult parasites. The protein count is based on a cut-off of at least 2 unique peptides