Fig. 3

Global pattern of 3’UTR and polyA processing signals. a the nucleotide composition near the polyA site at the single-base resolution. b the distribution of top hexamers 50 bp up- and down- stream of polyA site cluster under control (CK), cold (LT) and heat (HT) conditions. Blue arrows indicate the accumulation peak of “AAAAAA” downstream of PACs in all samples. Red arrow indicates the G-containing hexamer “ATTTTG” is missed under heat and cold stresses. Left y-axis indicates the counts of hexamers, and the right y-axis indicate the highlighted hexamer in yellow with black line. Yellow color highlights unique hexamer in samples: the “ATTTG” hexamer in CK, “ATTTAT” in LT, “ATTATT” in HT. c The identification of antisense PACs under control, cold and heat conditions. The star indicates a significant increase of antisense PACs under heat condition thais calculated by the right-sided Fisher’s extract test