Table 1 SSR primers for probe labeling
SSR motifs | Primers for probe labelling | FISH signals on wheat chromosomes | |
|---|---|---|---|
Forward primer (5′-′3) | Reverse primer (5′-′3) | ||
AAC | AACAACAACAACAACAACAACAACAACAAC | GTTGTTGTTGTTGTTGTTGTTGTTGTTGTT | Strong |
AAG | AAGAAGAAGAAGAAGAAGAAGAAGAAGAAG | CTTCTTCTTCTTCTTCTTCTTCTTCTTCTT | Strong |
AGC | AGCAGCAGCAGCAGCAGCAGCAGCAGCAGC | GCTGCTGCTGCTGCTGCTGCTGCTGCTGCT | Strong |
ACG | ACGACGACGACGACGACGACGACGACGACG | CGTCGTCGTCGTCGTCGTCGTCGTCGTCGT | Strong |
ACA | ACAACAACAACAACAACAACAACAACAACA | TGTTGTTGTTGTTGTTGTTGTTGTTGTTGT | Strong |
AG | AGAGAGAGAGAGAGAGAGAG | CTCTCTCTCTCTCTCTCTCT | Strong |
AGG | AGGAGGAGGAGGAGGAGGAGGAGGAGGAGG | CCTCCTCCTCCTCCTCCTCCTCCTCCTCCT | Weak |
ATC | ATCATCATCATCATCATCATCATCATCATC | GATGATGATGATGATGATGATGATGATGAT | Weak |
ACC | ACCACCACCACCACCACCACCACCACCACC | GGTGGTGGTGGTGGTGGTGGTGGTGGTGGT | Weak |
GT | GTGTGTGTGTGTGTGTGTGT | ACACACACACACACACACAC | None |
TA | TATATATATATATATATATA | TATATATATATATATATATA | None |
GC | GCGCGCGCGCGCGCGCGCGC | GCGCGCGCGCGCGCGCGCGC | None |
CGG | CGGCGGCGGCGGCGGCGGCGGCGGCGGCGG | CCGCCGCCGCCGCCGCCGCCGCCGCCGCCG | None |
GTC | GTCGTCGTCGTCGTCGTCGTCGTCGTCGTC | GACGACGACGACGACGACGACGACGACGAC | None |
GGA | GGAGGAGGAGGAGGAGGAGGAGGAGGAGGA | CCTCCTCCTCCTCCTCCTCCTCCTCCTCCT | None |
TCC | TTCTTCTTCTTCTTCTTCTTCTTCTTCTTC | GAAGAAGAAGAAGAAGAAGAAGAAGAAGAA | None |
GCG | GCGGCGGCGGCGGCGGCGGCGGCGGCGGCG | CGCCGCCGCCGCCGCCGCCGCCGCCGCCGC | None |
TCA | TCATCATCATCATCATCATCATCATCATCA | TGATGATGATGATGATGATGATGATGATGA | None |
GAG | GAGGAGGAGGAGGAGGAGGAGGAGGAGGAG | CTCCTCCTCCTCCTCCTCCTCCTCCTCCTC | None |
GCAC | GCACGCACGCACGCACGCACGCACGCACGCACGCACGCAC | GTGCGTGCGTCGTGCGTGCGTGCGTGCGTGCGTGCGTGC | None |
TAAT | TAATTAATTAATTAATTAATTAATTAATTAATTAATTAAT | ATTAATTAATTAATTAATTAATTAATTAATTAATTAATTA | None |