Regulatory roles of differentially expressed MicroRNAs in metabolic processes in negative Lens-induced myopia Guinea pigs

Table 3 Primer sequences for differentially expressed miRNAs determined by quantitative PCR

Target gene name	Primers
5S rRNA	F: 5’TCTCGTCTGATCTCGGAAGC3’
5S rRNA	R: 5’GCGGTCTCCCATCCAAGTA3’
cavPor3-miR-novel-chrscaffold-13-13,335	GSP: 5’CGATTCGTTGGCCTACAGAAGTG3’
cavPor3-miR-novel-chrscaffold-13-13,335	R: 5’ATCCAGTGCAGGGTCCGAGG3’
cavPor3-miR-novel-chrscaffold-120-37,436	GSP: 5’CGCTCCGAATGTGTAGCAGAAGA3’
cavPor3-miR-novel-chrscaffold-120-37,436	R: 5’ATCCAGTGCAGGGTCCGAGG3’
cavPor3-miR-novel-chrscaffold-119-37,316	GSP: 5’GGGGGTGGAATGTAAAGAAGT3’
cavPor3-miR-novel-chrscaffold-119-37,316	R: 5’GTGCGTGTCGTGGAGTCG3’
cavPor3-miR-novel-chrscaffold-128-37,706	GSP: 5’GGGGTTGTGCAAATCCATG3’
cavPor3-miR-novel-chrscaffold-128-37,706	R: 5’GTGCGTGTCGTGGAGTCG3’
cavPor3-miR-novel-chrscaffold-107-36,268	GSP: 5’CGCCATCGTAACACTGTCTGGTA3’
cavPor3-miR-novel-chrscaffold-107-36,268	R: 5’ATCCAGTGCAGGGTCCGAGG3’
cavPor3-miR-novel-chrscaffold-76-32,980	GSP: 5’CGACATTGCCTAAGCCAGGGATT3’
cavPor3-miR-novel-chrscaffold-76-32,980	R: 5’ATCCAGTGCAGGGTCCGAGG3’

GSP is the specific primer which is matched to target gene; R is the primer that is matched to reverse transcription primer (R)

ISSN: 1471-2164