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Table 3 qRT-PCR of specific pilin-associated genes to validate RNA-Seq analyses

From: Changes in the expression of genes encoding type IV pili-associated proteins are seen when Clostridium perfringens is grown in liquid or on surfaces

 

RNA-Seq fold changes

qRT-PCR ΔΔCt fold changes

BHI PL/LI

PGY PL/LI

FABG PL/LI

BHI PL/LI

PGY PL/LI

FABG PL/LI

pilA2 (cpe2284)

0.873*

1.326

2.339

1.21–1.69

1.07–2.05

1.33–2.93

pilB2 (cpe2286)

1.143

2.950

5.233

0.74–1.44

1.07–3.62

2.98–5.68

pilC2 (cpe2285)

0.807

3.063

4.178*

0.79–2.29

1.95–3.41

1.52–3.02

pilT (cpe1767)

0.614*

1.735

0.942

0.77–1.28

1.27–2.09

0.87–1.64

cpe2277

0.679*

3.128

1.699*

0.72–1.87

1.82–3.18

0.88–1.61

lon (cpe2635)

0.795

0.939

1.130

   
  1. *indicate a value out of fold change range but shows similar trends
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BMC Genomics

ISSN: 1471-2164

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