Fig. 8

Quantitative real-time PCR (qRT-PCR) analysis of six unigenes encoding enzymes involved in the triterpenoid biosynthesis pathway in C. gracile. a Un 41,982 encoding DXR. b CL1648-1 encoding HDR. c CL10352–1 encoding HMGR. d Un 5223 encoding HMGR. e Un 17, 275 encoding HMGR. f CL12163–4 encoding SS. Blue bars show the results of the qRT-PCR analysis, and red lines show the FPKM values determined by RNA-Seq analysis. The left Y-axis indicates the relative expression level of the qRT-PCR detection gene, and the right Y-axis indicates the FPKM value in the RNA-Seq data. F, flowers; L, leaves; S, stems; R, roots. Data represent mean ± standard error (SE; n = 3)