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Table 1 CDPK genes in B. distachyon

From: Genome-wide survey and expression analysis of calcium-dependent protein kinase (CDPK) in grass Brachypodium distachyon

Gene NameGene LocusGenebank Acc. No.ORF (bp)Exon No.Pepide lengthPKDEF-hand domainPIMW (KD)N- myristaN- PalmitbSubcellular Localizationc
BdCDPK01Bradi1g04440XM_0102296521602853358–316366–5336.3159.96NYpero: 6, mito: 5, chlo: 2
BdCDPK02Bradi1g06270XM_01023042817737590127–385435–5905.2964.59NYvacu: 6, chlo: 4, nucl: 2, pero: 1
BdCDPK03Bradi1g06300XM_02445666918727623157–415465–6235.8468.48NYchlo: 6, vacu: 6, nucl: 1
BdCDPK04Bradi1g12150XM_00355951618636620109–405455–6209.3568.74NYchlo: 6, vacu: 3, E.R.: 2, nucl: 1.5, cysk_nucl: 1.5
BdCDPK05Bradi1g24240XM_0035628251617853864–322372–5386.9860.5NYcyto: 7, pero: 3, E.R.: 2, plas: 1
BdCDPK06Bradi1g26310XM_0244566131599853284–342391–5325.859.38NYchlo: 8, cyto: 3, nucl: 2
BdCDPK07Bradi1g52567XM_00355725215511251653–313363–5167.6357.85NYcysk: 7, cyto: 3, chlo: 1, nucl: 1, plas: 1
BdCDPK08Bradi1g56970XM_01023006617047567105–363413–5675.1762.42NYE.R.: 5, chlo: 2, nucl: 2, cyto: 1, plas: 1, vacu: 1, pero: 1
BdCDPK09Bradi1g76560XM_0244566751653755080–339389–5505.4561.51NYnucl: 3.5, cyto_nucl: 3.5, mito: 3, plas: 3, cyto: 2.5, chlo: 2
BdCDPK10Bradi2g15520XM_0035677831647854896–354404–5486.5662.12YNchlo: 5, nucl: 5, pero: 3
BdCDPK11Bradi2g21390XM_0035681551551751662–320370–5165.5956.94NYchlo: 3, plas: 3, vacu: 2, E.R.: 2, nucl: 1.5, cysk_nucl: 1.5
BdCDPK12Bradi2g21790XM_0035660581575752413–298364–5245.3358NNchlo: 6, cyto: 4, nucl: 3.5, nucl_plas: 2.5
BdCDPK13Bradi2g22750XM_0035682181647754874–332382–5486.2560.79NYcyto: 4, mito: 3, chlo: 2, E.R.: 2, pero: 1, cysk: 1
BdCDPK14Bradi2g43910XM_0035693381581852674–332382–5265.859.44YNmito: 8, chlo: 4, nucl: 1
BdCDPK15Bradi2g52870XM_0035643901545651459–317367–5145.4556.78NYchlo: 4, plas: 3, nucl: 2.5, cyto: 2, cysk_nucl: 2, vacu: 1
BdCDPK16Bradi2g54080XM_0244588971686656162–320370–5616.4963.26NYchlo: 4, cyto: 4, mito: 2, E.R.: 1, pero: 1, cysk: 1
BdCDPK17Bradi3g02600XM_01023544615631252057–317367–5208.9958.64YYcyto: 7, chlo: 2, nucl: 2, mito: 2
BdCDPK18Bradi3g32187XM_0035719661527650866–324374–5085.2357.16NYmito: 4, nucl: 3, chlo: 2, cyto: 2, plas: 1, pero: 1
BdCDPK19Bradi3g41770XM_0035724211740357978–368418–5796.563.84YYchlo: 5, cyto: 2, mito: 2, nucl: 1, vacu: 1, E.R.: 1, cysk: 1
BdCDPK20Bradi3g51970XM_0035700701671755698–356406–5565.4556.78YYmito: 10.5, chlo_mito: 7.5, chlo: 3.5
BdCDPK21Bradi3g60750XM_0035706821599253257–325375–5325.1358.5YYcyto: 7, mito: 4, nucl: 2
BdCDPK22Bradi4g07280XM_01024064318336610158–416466–6105.9267.78YYchlo: 5, mito: 3.5, cyto_mito: 2.5, cyto: 2, nucl: 1, E.R.: 1
BdCDPK23Bradi4g24390XM_0035777351557751852–310360–5185.2857.05NNchlo: 11, E.R.: 2
BdCDPK24Bradi4g26317XM_0035762981599553275–333383–5325.658.44YYcyto: 7, chlo: 4, nucl: 2
BdCDPK25Bradi4g35100XM_0035783861698856588–358426–5655.9562.54YYchlo: 3, mito: 3, cyto: 2, plas: 2, vacu: 2, nucl: 1
BdCDPK26Bradi4g39870XM_0035787091713657081–339389–5706.1463.3NYchlo: 6, mito: 6, cyto: 1
BdCDPK27Bradi4g40300XM_0035787411572752348–306356–5235.5357.65NNchlo: 7, E.R.: 3, plas: 2, nucl: 1
BdCDPK28Bradi4g43400XM_0035789161650654991–349399–5494.9460.07NYcyto: 10, nucl: 3
BdCDPK29Bradi5g18250XM_0102419511719757299–388437–5726.664.05YYcyto: 7, chlo: 4, nucl: 2
BdCDPK30Bradi5g19430XM_0035803491686756198–356406–5615.4961.59YYmito: 9.5, chlo_mito: 7, chlo: 3.5
  1. ORF Open Reading Frame, PKD Protein kinase domain, PI isoelectric point
  2. a. The myristoylation site was predicted by Myristoylator program in ExPASy (http://web.expasy.org/myristoylator/)
  3. b. The palmitoylation site was predicted by CSS-plam4.0 (http://csspalm.biocuckoo.org/)
  4. c. chlo Chloroplast, cysk Cytoskeleton, cyto Cytoplasmic, E.R. Endoplasmic reticulum, mito Mitochondrial, nucl Nuclear, pero Peroxysome, plas Plasma membrane, vacu Vacuole