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Fig. 5 | BMC Genomics

Fig. 5

From: Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)

Fig. 5

Validation of selected genes using real-time PCR during sex reversal. 0w, the female grouper before MT implantation; 1w, the early stage of sex reversal after 1week implantation; 2w, the middle stage of sex reversal after 2 weeks implantation; 3w, the late stage of sex reversal after 3 weeks MT implantation. The data was shown as mean ± SEM (n = 4 or 5) and the values with different asterisks were significantly different with a probability level <0.05 (P < 0.05). Ef1a was used as the reference gene. a nr0b1; b erα; c erβ; d wnt9; e gdf9; f bmp15; g cyp17a1; h zbtb40

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