Table 2 The primers used in real-time PCR
Primers | Purpose | Sequence (from 5’to 3′) |
|---|---|---|
cyp17a1-F | real-time PCR | GGTATTACGGATCCGCCCTG |
cyp17a1-R | real-time PCR | AGGTAGCTGGGTGATGGGAT |
3hd-F | real-time PCR | GTACTGCGGTCAGGTTGGAA |
3hd-R | real-time PCR | CTCCAGGCACAGGGTTGTAG |
sdr12-F | real-time PCR | TAACAATGCTGGCTGTATGGTGAA |
sdr12-R | real-time PCR | CTTCTTCAGTGCGGGTATCAGC |
sdr11-F | real-time PCR | GAAAGACCGAGGGCTGGAG |
sdr11-R | real-time PCR | CGATGCCCACCCATACTGTT |
p5cdh-F | real-time PCR | AGCTGATGGTCCAGTGTTCG |
p5cdh-R | real-time PCR | GTTCTTCCCTCCGCACTCTC |
cyp3A40-F | real-time PCR | GCCTGAATGGAGACCTCTATGA |
cyp3A40-R | real-time PCR | GGGGAAACACCCTTGGAACA |
sdr13-F | real-time PCR | TGAGGCTGAATGGGAAGACG |
sdr13-R | real-time PCR | CACACCTGCGTTGTTGATGAG |
nr0b1-F | real-time PCR | CCAAGGAGTATGCGTATCTGAAA |
nr0b1-R | real-time PCR | CGTTGAGAGCCTGGTGCG |
era-F | real-time PCR | CTGCTCTCACACATCAGGCA |
era-R | real-time PCR | TGATTTGTCAGGCCGTTGGA |
erb-F | real-time PCR | CCACGGGACATTGCCTTAC |
erb-R | real-time PCR | GCTCTTACGGCGGTTCTTGT |
wnt9-F | real-time PCR | TGAGAACACCTGGCTCCAAC |
wnt9-R | real-time PCR | CAGATGACTCTGACCAGGCG |
gdf9-F | real-time PCR | CACAGTCAACGCAGAAAGGC |
gdf9-R | real-time PCR | GACCAGAAACCTCTGGTGGG |
bmp15-F | real-time PCR | ACCAGCACACCTAAGAACCG |
bmp15-R | real-time PCR | GAATGGAAGCCGTAGGGGAG |
zbtb40-Q-F | real-time PCR | ACCTTTGCTCACCCATCAGG |
zbtb40-Q-R | real-time PCR | AATGGACTGGGCGAAGACAG |
zbtb40-F3 | semi-qPCR | TTTCACCAAACAGGGACGACT |
zbtb40-R3 | semi-qPCR | CCTCTGCGTCTTTACACCCATT |