Fig. 4

Performance of 3’Pool-seq with low RNA input samples. a Number of genes detected (TPM > 1) when different RNA input amounts were used. b Correlations of ERCC spike-ins among replicates when different amounts of RNA input were used and ERCC spike-ins were diluted proportionately. c Comparisons of log₂(Fold-Changes) for DE genes (defined as FDR q-value< 0.05, log₂(Fold-Change) > 1 in the 3’Pool-seq run with 50 ng RNA input) between 10 ng input RNA 3’Pool-seq run and 50 ng input RNA 3’Pool-seq run