Fig. 4

Library pooling yields near even distribution of percent of the total reads: Optimal pooling is the expectation (1.04) of the percent reads if all 96 libraries pooled evenly. 4 unique plasmids of Nextera library prepared plasmids (a), 4 two-fold dilutions starting at 500 pg of AL library prepared gDNA (b). The bars are colored by the end RFU values of the qPCR, blue if the RFU was greater than 1000, yellow if the RFU was between 700 and 1000, and red if the RFU was less than 700. For gDNA library (b), samples below RFU 700 were likely to be underpooled because they were out of the linear range of the qPCR. This trend was not observed with the plasmid library (a)