Fig. 2

Analysis of splice junctions. a Distribution of splicing junctions in the annotated loci. b Distribution of splice events and splice junction levels. c Percentages of splicing donor-acceptor di-nucleotide usages among all transcripts. d Sequence logos of intronic 5′ splice sites, branch sites, and 3′ splice sites; logos were created using WebLogo 3. e Distribution of lengths of introns based on RNA-Seq (lengths of introns ≤2000 bp). f Relationship between branch point A offset from 5′splice site and intron length. Splice junction: multiple splicing events spanning the same intron were considered as one splicing junction. Annotated (known): The junction is part of the gene model. Both splice sites, 5′ splice site (5′SS) and 3′splice site (3’SS) are annotated by reference gene model. Complete_novel: Both 5′SS and 3′SS are novel. Partial_novel: One of the splice site (5′SS or 3′SS) is novel, and the other splice site is annotated