Zebrafish genome editing at three other target sites by zLOST
a Restriction enzyme-based method design of three target sites. Target sequence (black), PAM region (blue), target modification sites (red), and restriction site (underlined) are indicated. b Restriction enzymes are used to digest the amplified region of the target genes. T = th, N = nop56, R = rps14. The “positive embryos” groups are highlighted by asterisk. c Sequencing results of the th, nop56 and rps14 loci. Patterns of DNA modification observed in independent embryos pool. Note: △1 and △2 mean the presence of additional undesirable mutations outside of the shown sequence window.