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Fig. 3 | BMC Genomics

Fig. 3

From: CRISPR/Cas9-mediated precise genome modification by a long ssDNA template in zebrafish

Fig. 3

Zebrafish genome editing at three other target sites by zLOST

a Restriction enzyme-based method design of three target sites. Target sequence (black), PAM region (blue), target modification sites (red), and restriction site (underlined) are indicated. b Restriction enzymes are used to digest the amplified region of the target genes. T = th, N = nop56, R = rps14. The “positive embryos” groups are highlighted by asterisk. c Sequencing results of the th, nop56 and rps14 loci. Patterns of DNA modification observed in independent embryos pool. Note: 1 and 2 mean the presence of additional undesirable mutations outside of the shown sequence window.

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