Table 7 The numerical and biological outcomes of the multiple criteria approach we applied to resolve the impact of contaminating LD muscle on the IMF samples. The background list was composed of 34,228 genes including duplicates (14,477 unique). This approach eliminates the enrichment for ‘muscle systems process’ indicating the impact of the contaminating LD muscle in the IMF samples has been adequately dealt with
Group Query (in brackets number of genes satisfying individual criteria) | Number of genes satisfying criteria (in brackets duplicates removed) / % of total | Deviation from null expectation | Functional enrichment for ‘muscle systems process’ (FDR q value) |
|---|---|---|---|
IMF > 1.32 fold higher than the average of all fat depots (1833) AND IMF > 2 fold higher than LD (329) | 67 (49) / 0.196 | 3.82 fold enrichment compared to what would be expected if the two criteria were independent | None identified. No other hits at FDR q < 0.01 |
IMF > 1.68 fold higher than the average of all fat depots (741) AND IMF > LD by any amount (22,156) | 105 (76) / 0.307 | 4.52 fold depletion compared to what would be expected if the two criteria were independent | None identified. No other hits at FDR q < 0.01 |
IMF > 2 fold higher than SC (600) AND IMF > LD by any amount (22,156) | 93 (73) / 0.271 | 4.87 fold depletion compared to what would be expected if the two criteria were independent | None identified. No other hits at FDR q < 0.01 |
IMF > 1.32 fold lower than SC (890) AND IMF < LD by any amount (12,072) | 49 (41) / 0.143 | 6.41 depletion compared to what would be expected if the two criteria were independent | None identified. No other hits at FDR q < 0.01 |