Gene expression identifies metabolic and functional differences between intramuscular and subcutaneous adipocytes in cattle

Table 9 Expression profiles of particular isoforms in esterification and glycerolipid synthesis where IMF shows higher expression than SC that is not attributable to LD contamination. Normalised mean expression values (log2) for LD muscle, dissected IMF and SC. P values are reported for both DE and PIF (based on the SC versus IMF comparison). These non significant P values need to be interpreted cautiously as these genes do satisfy the independent requirement of higher expression in IMF than LD which we argue makes them potentially noteworthy

Pathway	Enzyme	Gene	Probe	Cellular localisation	LD	IMF	SC	IMF vs SC FC	IMF minus SC (log2)	P value (DE / PIF)
Esterification	Glycerol kinase 2; EC 2.7.1.30	GK2	A_73_113526	Cytoplasm, mitochondrion	5.83	6.20	5.90	1.23	0.30	0.28 / 0.41
Esterification	Glycerol kinase 5; EC 2.7.1.30	GK5	A_73_113437	As above	5.32	5.81	5.47	1.27	0.35	0.24 / 0.39
Esterification	1-acylglycerol-3-phosphate O-acyltransferase 1; EC 2.3.1.51	AGPAT1	A_73_110232	Endoplasmic reticuluum	9.67	10.44	10.03	1.33	0.42	0.18 / 0.20
Esterification	1-acylglycerol-3-phosphate O-acyltransferase 4; EC 2.3.1.51	AGPAT4	A_73_115012	Endoplasmic reticuluum, membrane	8.07	8.70	8.47	1.17	0.23	0.35 / 0.39
Esterification	1-acylglycerol-3-phosphate O-acyltransferase 9; EC 2.3.1.51	AGPAT9	A_73_P272036	Endoplasmic reticuluum, membrane	11.55	12.14	11.63	1.43	0.51	0.11 / 0.10
Glycerolipid synthesis	membrane bound O-acyltransferase domain containing 1; EC 2.3.1.n6	MBOAT1	A_73_108662	Endoplasmic reticuluum, membrane	8.57	9.56	9.20	1.28	0.36	0.22 / 0.27
Glycerolipid synthesis	membrane bound O-acyltransferase domain containing 2 EC 2.3.1.n7, 2.3.1.51	MBOAT2	A_73_118887	Endoplasmic reticuluum, membrane	5.23	5.68	5.14	1.46	0.55	0.10 / 0.30
Glycerolipid synthesis	Acylglycerol kinase; EC 2.7.1.107, 2.7.1.94	AGK	A_73_102007	Mitochondrion	4.28	4.58	4.26	1.25	0.32	0.26 / 0.45

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