Fig. 5

Mammalian genomes contain multiple Zmat2 pseudogenes. a. Schematic of the two Zmat2 pseudogenes in the microbat genome. The color-coding indicates regions of each pseudogene that are similar in DNA sequence to individual coding segments of authentic Zmat2 (red – exon 2; blue – exon 3; yellow – exon 4; green – exon 5; pink – coding region of exon 6). The white areas depict segments similar to the 3′ UTR of authentic Zmat2 exon 6 in each pseudogene. A scale bar is shown. b. Alignment of amino acid sequences of microbat ZMAT2 and the predicted pseudogene protein (Z1). Similarities and differences are shown, with identities being indicated by asterisks. Differences are marked in red text. The blue text denotes the two amino acids that are different from mouse or human ZMAT2 (also see Fig. 7). c. Schematic of the four Zmat2 pseudogenes in the dolphin genome. The color-coding indicates regions of each pseudogene that are similar in DNA sequence to individual exons of authentic Zmat2, as per part a above, and the white areas depict segments similar to the 3′ UTR of authentic Zmat2 exon 6 in each pseudogene. A scale bar is shown. d. Alignment of amino acid sequences of dolphin ZMAT2 and predicted pseudogene proteins (Z1 and Z3). Similarities and differences are shown, with identities being indicated by asterisks. Differences also are marked in red text. e. Phylogenetic tree of mammalian Zmat2 pseudogenes. The data on marmoset are from (Baral K, Rotwein P: The story of ZMAT2: a highly conserved and understudied human gene, manuscript submitted). The scale bar indicates 0.01 substitutions per site and the length of each branch approximates the evolutionary distance