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Table 1 Sampling site, mycorrhiza, and analyses performed. All fruiting bodies reached stage 5 of maturation [20], as described in Methods section

From: Tuberomics: a molecular profiling for the adaption of edible fungi (Tuber magnatum Pico) to different natural environments

 

Site (Province)

Region

Mycorrhiza

Years of analysis

Period

Sample namesa

Total number of samplesb

Proteomics

Alba (CN)

Piedmont

Poplar (Populus alba)

4

2012–2015

AL 2012

AL 2013

AL 2014

AL 2015

24

Isernia (IS)

Molise

Poplar (Populus alba)

3

2013–2015

IS 2013

IS 2014

IS 2015

18

San Miniato (PI)

Tuscany

n. s. (Wood)

4

2012–2015

SM 2012

SM 2013

SM 2014

SM 2015

24

RNAseq / qPCR

Alba (CN)

Piedmont

Poplar (Populus alba)

2

2014–2015

AL 2014

AL 2015

12

Isernia (IS)

Molise

Poplar (Populus alba)

2

2014–2015

IS 2014

IS 2015

12

San Miniato (PI)

Tuscany

n. s. (Wood)

2

2014–2015

SM 2014

SM 2015

12

PTR-ToF-MS analysis

Alba (CN)

Piedmont

Poplar (Populus alba)

4

2014–2017

AL 2014

AL 2015

AL 2016

AL 2017

27

Isernia (IS)

Molise

Poplar (Populus alba)

4

2014–2017

IS 2014

IS 2015

IS 2016

IS 2017

24

San Miniato (PI)

Tuscany

n. s. (Wood)

4

2014–2017

SM 2014

SM 2015

SM 2016

SM 2017

41

GC-MS analysis

Alba (CN)

Piedmont

Poplar (Populus alba)

4

2014–2017

AL 2014

AL 2015

AL 2016

AL 2017

23

Isernia (IS)

Molise

Poplar (Populus alba)

4

2014–2017

IS 2014

IS 2015

IS 2016

IS 2017

18

San Miniato (PI)

Tuscany

n. s. (Wood)

4

2014–2017

SM 2014

SM 2015

SM 2016

SM 2017

23

  1. a As reported in the figure and in the main text
  2. b The total number of analyzed samples during different years. Six independent biological replicates were analyzed for each sample over years for proteomic and molecular analysis. Five replicates were analyzed for VOCs analysis (GC-MS and PTR-ToF) for each accession during the first 2 years (2014–2015), whereas a variable number of samples (from four to fifteen) were analyzed during the remaining 2 years (2016–2017)