Fig. 7

This “Lipid Metabolism” network (Panel a) is centered on interactions of three transcription factors, thyroid hormone-responsive Spot14 protein (THRSP), sterol response element binding factor 2 (SREBF2) and nuclear factor, erythroid 2-like 2 (NFE2L2) and their direct target genes from the D2FAST48h vs. D2REFED4h contrast. IPA predicted activation (orange lines and arrows) or inhibition (blue lines) of direct targets of SREBF2 identified in the D2FAST48h vs. D2REFED4h contrast (Panel b). Of the 22 direct target genes identified, only six AR-DEGs were expressed at higher levels in liver of D2FAST48h chicks. As such, Ingenuity predicts that SREBF2 should be inhibited (blue symbol), which would lead to inhibition (blue arrows/edges) of 16 DEGs (green symbols) that control lipogenesis under the direction of the most highly-expressed gene in liver of fed or refed cockerels— the lipogenic transcription factor THRSPA