Fig. 2

Venom characterization for Bothriechis nigroviridis. a Venom transcriptome compositions for B. nigroviridis based on average expression between two individuals. b Venom transcriptome compositions of each individual used. The venom of B. nigroviridis CLP1864 is largely consistent with the published proteome for this species. The high proportion of snake venom metalloproteinases (SVMPs) observed in the venom gland transcriptome of B. nigroviridis CLP1856 has not been described previously. c Intraspecific variation in transcript expression for B. nigroviridis. Data have been centered log-ratio transformed to account for their compositional nature. Dashed lines denote the 99% confidence interval of nontoxin expression and red lines are lines of best fit based on orthogonal residuals. B. nigroviridis displays substantially more variation in toxin expression, primarily in C-type lectins (CTLs), SVMPs, and snake venom serine proteinases (SVSP)s