Fig. 13

Illustration of sample to sequence workflow and estimated times for each step. Sample preparation, extraction, PCR, library preparation, and sequencing work flows withestimated times are shown. Both the PCR workflow (top) and sequencing workflow (bottom) include preparing and extracting samples in batches of 45 (9x) and 32 (3x) samples in parallel. In this study, a total of 12 ONT flow cells were used to sequence 513 samples. See Table 1 for a detailed breakdown of sample sets. See Table 2 for a list of spike concentrations. Clip art, photo and screenshots depicted are from the authors