Fig. 8

Real-time PCR receiver operating characteristic (ROC) curves. Curves are shown for each of the 14 assays tested (faceted by column), split over the 5 steps of the dilution series (faceted by rows). Replicates are kept separated for this analysis. The Ct value cutoff for calling positive/negative is ranged from 0 to 40 in steps of 0.1. The area under the curve (AUC) is calculated using the ‘step’ method. Most assays achieve a 100% sensitivity and 0% FPR at the standard Ct value cutoff of 40. The FPR for all assays remains at or very near zero across all cutoffs