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Table 2 Target Concentration (CFU/ml) of spiked materials for Set 1 and Set 2

From: Comparison of the performance of an amplicon sequencing assay based on Oxford Nanopore technology to real-time PCR assays for detecting bacterial biodefense pathogens

Set

Step

Strain

239

240

241

113

114

164

197

708-gi

708-live

1

5

1.25E+ 05

2.50E+ 04

9.53E+ 04

2.50E+ 05

2.50E+ 05

5.00E+ 05

5.00E+ 05

2.50E+ 05

2.50E+ 05

4

1.25E+ 04

2.50E+ 03

9.53E+ 03

2.50E+ 04

2.50E+ 04

5.00E+ 04

5.00E+ 04

2.50E+ 04

2.50E+ 04

3

1.25E+ 03

2.50E+ 02

9.53E+ 02

2.50E+ 03

2.50E+ 03

5.00E+ 03

5.00E+ 03

2.50E+ 03

2.50E+ 03

2

1.25E+ 02

2.50E+ 01

9.53E+ 01

2.50E+ 02

2.50E+ 02

5.00E+ 02

5.00E+ 02

2.50E+ 02

2.50E+ 02

1

1.30E+ 01

2.50E+ 00

9.53E+ 00

2.50E+ 01

2.50E+ 01

5.00E+ 01

5.00E+ 01

2.50E+ 01

2.50E+ 01

2

5

1.25E+ 05

2.50E+ 04

9.53E+ 04

2.50E+ 05

2.50E+ 05

5.00E+ 05

5.00E+ 05

2.50E+ 05

n/a

4

1.25E+ 04

2.50E+ 03

9.53E+ 03

2.50E+ 04

2.50E+ 04

5.00E+ 04

5.00E+ 04

2.50E+ 04

n/a

3

1.25E+ 03

2.50E+ 02

9.53E+ 02

2.50E+ 03

2.50E+ 03

5.00E+ 03

5.00E+ 03

2.50E+ 03

n/a

2

1.25E+ 02

2.50E+ 01

9.53E+ 01

2.50E+ 02

2.50E+ 02

5.00E+ 02

5.00E+ 02

2.50E+ 02

n/a

  1. Concentrations were selected to ensure consistent detection by real-time PCR (Ct < 30) at the highest concentration(s). The “Step” column indicates position within the serial 10 fold dilution sequence. n/a: not applicable
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BMC Genomics

ISSN: 1471-2164

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