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Table 6 Raw read counts (and percent read counts) for false positive assays

From: Comparison of the performance of an amplicon sequencing assay based on Oxford Nanopore technology to real-time PCR assays for detecting bacterial biodefense pathogens

Organism

Strain

PCR Assay Number

23

28

29

30

07

11

50

F. tularensis

239

  

8917 (0.2842%)

23,584 (0.7516%)

   

F. tularensis

240

   

30,616 (1.0196%)

24 (0.0008%)

  

F. tularensis

241

  

11,037 (0.3579%)

 

59 (0.0019%)

  

Y. pestis

113

  

22 (0.0009%)

    

Y. pestis

114

25 (0.0025%)

51 (0.0025%)

3 (0.0001%)

  

3417 (0.1655%)

 

B. mallei

164

      

4 (0.0093%)

  1. Sample read counts combined across concentrations and conditions to give total FP count and percentage per assay